SediTox Direct™ Toxicity Sediment Test

The direct contact sediment toxicity assay is based on the ability of a toxicant to inhibit the de novo synthesis of an inducible enzyme (β-galactosidase) in a highly permeable mutant of E. coli and the results manifest as an easily observable colour change after a short incubation period.

SediTox™ Qualitative kit includes:

1. Reaction mixture - a cocktail containing an enzymatic inducer and cofactors required for the recovery of test bacteria from their stressed condition.
2. lyophilized bacteria - a highly permeable selected mutant of E. coli. (1 vial)
3. Rehydration Solution (1 vial)
4. Positive control HgCl2 1 mL (1 vial)
5. Chromogen (1 vial)
6. SediTox™ diluent 10 mL (4 vials)

Plastics:

• Plastics and additional components:
• Disposable plastic pipets. (3 pipets)
• Disposable test tubes. (25 tubes)
• Disposable SPT filter cartridges (25 units)
• Sterile 96-well microplate. (1 unit)
• Reference Soil (1 unit)

SediTox™ Quantitative Calculation kit includes:

1. Quantitative Reagents

Plastics:

• Plastics and additional components:
• Disposable plastic pipets. (3 pipets)
• Disposable test tubes. (25 tubes)
• Disposable SPT filter cartridges (25 units)
• Sterile 96-well microplate. (1 unit)
• Reference Soil (1 unit)
• Disposable test tubes (25 tubes)
• Disposable 0.45 µm syringe filters (3 units)
• Disposable 10 ml syringes (3 units)
• Sterile 96-well microplate. (1 unit)